abstract：:Currently, there is no single method to collect, process, and analyze a water sample for all pathogenic microorganisms of interest. Some of the difficulties in developing a universal method include the physical differences between the major pathogen groups (viruses, bacteria, protozoa), efficiently concentrating large...

journal_title：Journal of microbiological methods

authors： Straub TM,Chandler DP

更新日期：2003-05-01 00:00:00

abstract：:Cyclospora cayetanensis, a coccidian parasite, with a fecal-oral life cycle, has become recognized worldwide as an emerging human pathogen. Clinical manifestations include prolonged gastroenteritis. While most cases of infection with C. cayetanensis in the United States have been associated with foodborne transmission...

journal_title：Journal of microbiological methods

authors： Varma M,Hester JD,Schaefer FW 3rd,Ware MW,Lindquist HD

更新日期：2003-04-01 00:00:00

abstract：:To develop a quantitative assay of fungal growth inside plant tissues, strains of Colletotrichum destructivum and Colletotrichum orbiculare were transformed with a modified green fluorescent protein (GFP) gene fused with a glyceraldehyde-3-phosphate dehydrogenase promoter from Aspergillus nidulans. Transformants expre...

journal_title：Journal of microbiological methods

authors： Chen N,Hsiang T,Goodwin PH

更新日期：2003-04-01 00:00:00

abstract：:This paper describes a method that facilitates the extraction of PCR-compatible DNA from different activated sludge samples. The approach involves a novel preprocessing step in DNA extraction, which removes potential PCR inhibitors. The sludge was washed with different ratios of acetone and petroleum ether after pretr...

journal_title：Journal of microbiological methods

authors： Purohit HJ,Kapley A,Moharikar AA,Narde G

更新日期：2003-03-01 00:00:00

abstract：:The detection and molecular typing of Helicobacter pylori virulence genes in human stool specimens by polymerase chain reaction (PCR) require an adequate amount of bacterial DNA and an appropriately adjusted PCR protocol. DNA was isolated from stool samples of 39 H. pylori-infected and nine uninfected Colombian childr...

journal_title：Journal of microbiological methods

authors： Sicinschi LA,Correa P,Bravo LE,Schneider BG

更新日期：2003-02-01 00:00:00

abstract：:Real-time TaqMan-PCR assays were developed for detection, differentiation and absolute quantification of the pathogenic subspecies of Clavibacter michiganensis (Cm) in one single PCR run. The designed primer pair, targeting intergenic sequences of the rRNA operon (ITS) common in all subspecies, was suitable for the am...

journal_title：Journal of microbiological methods

authors： Bach HJ,Jessen I,Schloter M,Munch JC

更新日期：2003-01-01 00:00:00

abstract：:Checkerboard DNA-DNA hybridisation enabled the quantitative analysis of plaque samples against 40 microbial species simultaneously, using digoxygenin-labelled, whole-genome DNA probes. This technique was initially developed to study the predominantly Gram-negative sub-gingival plaque microbiota. The aim of this study ...

journal_title：Journal of microbiological methods

authors： Wall-Manning GM,Sissons CH,Anderson SA,Lee M

更新日期：2002-11-01 00:00:00

abstract：:Effective biolistic transformation of intact conidia from the filamentous fungus Trichoderma reesei was achieved using the Bio-Rad Hepta Adaptor system with seven barrels for particle launch. Transformation frequencies of up to 39 colonies per microg of circular DNA and 37 colonies per microg of linear DNA were obtain...

journal_title：Journal of microbiological methods

authors： Te'o VS,Bergquist PL,Nevalainen KM

更新日期：2002-11-01 00:00:00

abstract：:A real-time PCR (RTm-PCR) assay using fluorescently labeled oligonucleotides (TaqMan probes) was used to detect and quantify the recombinant Rhodococcus sp. strain RHA1(fcb) in soil. One primer and probe set targeted a hypervariable region of the 16S rRNA gene unique to strain RHA1(fcb) and its phylogenetic relatives,...

journal_title：Journal of microbiological methods

authors： Rodrigues JL,Aiello MR,Urbance JW,Tsoi TV,Tiedje JM

更新日期：2002-10-01 00:00:00

abstract：:Aminotransferases (ATs) had previously been detected after native electrophoresis. We show now that aminotransferase(s) of Propionibacterium freudenreichii can be detected after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Moreover, it retained a high activity (84%) in the presence of 0.23% S...

journal_title：Journal of microbiological methods

authors： Thierry A,Maillard MB,Lortal S

更新日期：2002-09-01 00:00:00

abstract：:We developed an immunocapture-based polymerase chain reaction (PCR) assay for simultaneous detection of Cryptosporidium parvum oocysts and Giardia intestinalis cysts in surface water. Using primer pairs Cry9/Cry15 and LaxA/LaxB for Cryptosporidium and Gdh1/Gdh4 for Giardia, the sensitivity of the entire detection proc...

journal_title：Journal of microbiological methods

authors： Rimhanen-Finne R,Hörman A,Ronkainen P,Hänninen ML

更新日期：2002-08-01 00:00:00

abstract：:This paper describes a simple method for the isolation of salt-tolerant myxobacteria from marine conditions. As the results show in this paper, salt-tolerant myxobacteria are found to be able to grow, but unable to form fruiting bodies at high salt concentrations. The fruiting body structures of the salt-tolerant stra...

journal_title：Journal of microbiological methods

authors： Li YZ,Hu W,Zhang YQ,Qiu Zj,Zhang Y,Wu BH

更新日期：2002-07-01 00:00:00

abstract：:The ideal ecological metabolic activity assay would be applied to naturally occurring microbial populations immediately fixed in the field, and the assay would focus upon intracellular parameters indicative of a dynamic biogeochemical process. In this study, fluorescent in situ hybridization (FISH) with tyramide signa...

journal_title：Journal of microbiological methods

authors： Bakermans C,Madsen EL

更新日期：2002-06-01 00:00:00

abstract：:Analysis of microbial community structure in complex environmental samples using nucleic acid techniques requires efficient unbiased DNA extraction procedures; however, humic acids and other contaminants complicate the isolation of PCR-amplifiable DNA from compost and other organic-rich samples. In this study, combina...

journal_title：Journal of microbiological methods

authors： LaMontagne MG,Michel FC Jr,Holden PA,Reddy CA

更新日期：2002-05-01 00:00:00

abstract：:An integrative plasmid containing a 1.3 kb fragment of chromosomal DNA from Enterobacter amnigenus was constructed. The Omega fragment encoding spectinomycin/streptomycin resistance was cloned into the unique BglII site of the resulting plasmid, and the interrupted fragment was transferred via plasmid pMAK705 by elect...

journal_title：Journal of microbiological methods

authors： Nantapong N,Tanapongpipat S,Cole J,Panyim S

更新日期：2002-05-01 00:00:00

abstract：:An etiological relationship between periodontitis, a significant oral health problem, and the anaerobe Porphyromonas gingivalis may be related to the expression of a variety of putative virulence factors. The objective of the experiments described here was to develop a quantitative reverse transcription polymerase cha...

journal_title：Journal of microbiological methods

authors： Shelburne CE,Gleason RM,Germaine GR,Wolff LF,Mullally BH,Coulter WA,Lopatin DE

更新日期：2002-04-01 00:00:00

abstract：:A temperature-gradient incubator (TGI) is described, which produces a thermal gradient over 34 aluminium modules (15x30x5 cm) intersected by 2-mm layers of partly insulating graphite foil (SigraFlex Universal). The new, sandwich-designed TGI has 30 rows of six replicate sample wells for incubation of 28-ml test tubes....

journal_title：Journal of microbiological methods

authors： Elsgaard L,Jørgensen LW

更新日期：2002-03-01 00:00:00

abstract：:Recently, we demonstrated that a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) can be used to determine the molecular weight of polymerase chain reaction (PCR) products of intact 16S rRNA regions and to profile their restriction digests. This is the first time that MALDI-T...

journal_title：Journal of microbiological methods

authors： Taranenko NI,Hurt R,Zhou JZ,Isola NR,Huang H,Lee SH,Chen CH

更新日期：2002-02-01 00:00:00

abstract：:Cyanobacteria are an ancient and diverse group of photosynthetic microorganisms, which inhabit many different and extreme environments. This indicates a high degree of biological adaptation, which has enabled these organisms to thrive and compete effectively in nature. The filamentous cyanobacterium, Lyngbya majuscula...

journal_title：Journal of microbiological methods

authors： Burja AM,Abou-Mansour E,Banaigs B,Payri C,Burgess JG,Wright PC

更新日期：2002-02-01 00:00:00

abstract：:Staphylococcus aureus is a bacterial species with pathogenic potential to both humans and animals. The primary natural niche is said to be the human vestibulum nasi from where bacterial cells may spread to the environment or additional anatomical sites such as the perineum or the hands, where residence is usually tran...

journal_title：Journal of microbiological methods

authors： Van Belkum A,Kools-Sijmons M,Verbrugh H

更新日期：2002-01-01 00:00:00

abstract：:Tropheryma whipplei is the causative agent of Whipple's disease (WD), a chronic, life-threatening infection. Laboratory diagnosis is mainly based on PCR and histopathological analysis in duodenal biopsies and other specimens requiring invasive procedures. We have examined the presence of antibodies to recombinant heat...

journal_title：Journal of microbiological methods

authors： Morgenegg S,Maibach R,Chaperon DN,Herzog K,Altwegg M

更新日期：2001-12-01 00:00:00

abstract：:A novel method for the measurement of dissolved adenosine-5'-triphosphate and guanosine-5'-triphosphate (D-ATP and D-GTP, respectively) in marine and freshwater habitats was developed and applied to samples collected from the oligotrophic North Pacific Ocean. Both D-ATP and D-GTP are co-precipitated by authigenically ...

journal_title：Journal of microbiological methods

authors： Björkman KM,Karl DM

更新日期：2001-11-01 00:00:00

abstract：:DNA extracts from sediment and water samples are often contaminated with coextracted humic-like impurities. Estuarine humic substances and vascular plant extract were used to evaluate the effect of the presence of such impurities on DNA hybridization and quantification. The presence of humic substances and vascular pl...

journal_title：Journal of microbiological methods

authors： Bachoon DS,Otero E,Hodson RE

更新日期：2001-10-01 00:00:00

abstract：:Fermentation of alpha-glucosides (maltose, maltotriose) by Saccharomyces cerevisiae cells is a critical phase in the processes of brewing and breadmaking. Utilization of alpha-glucosides requires the active transport of the sugar across the cell membrane and, subsequently, its hydrolysis by cytoplasmic glucosidases. A...

journal_title：Journal of microbiological methods

authors： Hollatz C,Stambuk BU

更新日期：2001-09-01 00:00:00

abstract：:A pair of polymerase chain reaction (PCR) primers was designed on the basis of the nucleotide sequence homology of dextranase genes (dex) of Streptococcus mutans, S. sobrinus and S. downei. The primer pair amplified a 530-bp DNA fragment on the dex genes of mutans streptococcal species: S. mutans, S. sobrinus, S. down...

journal_title：Journal of microbiological methods

authors： Igarashi T,Ichikawa K,Yamamoto A,Goto N

更新日期：2001-08-01 00:00:00

abstract：:The fluorescent redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), combined with fluorescein-labeled antibodies, was tested for the simultaneous detection of the respiratory electron transport system (ETS) activity and enumeration of Giardia cysts and Cryptosporidium oocysts by spectral microfluorometry and epi...

journal_title：Journal of microbiological methods

authors： Iturriaga R,Zhang S,Sonek GJ,Stibbs H

更新日期：2001-07-30 00:00:00

abstract：:Specific fatty acids from phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) recovered from a per 13C-labeled bacteria can be detected in environmental samples and used as measures of bacterial transport in the subsurface. Detection of palmitic acid (16:0) and oleic acid (18:1) at m/z 271 (255+16) and 299 (28...

journal_title：Journal of microbiological methods

authors： Lytle CA,Fuller ME,Gan YM,Peacock A,DeFlaun MF,Onstott TC,White DC

更新日期：2001-04-01 00:00:00

abstract：:Direct comparisons are made between covalently linked streptavidin and silver ion coated microplates. Both coatings can immobilize biotinylated molecules. Silver ion coated microplate wells can immobilize 1.8 times higher amounts of biotin labeled horseradish peroxidase. The quantitation range and capacity for the cap...

journal_title：Journal of microbiological methods

authors： Bonen MR,García AA,Hoffman SA

更新日期：2001-03-01 00:00:00

abstract：:Silver scurf caused by Helminthosporium solani causes significant economic losses in table stock, seed and processing potatoes. Specific polymerase chain reaction (PCR) primers, Hs1F1/Hs2R1, from H. solani were used for the amplification of a 447-bp product from 20 tissue samples and 54 single spore H. solani isolates...

journal_title：Journal of microbiological methods

authors： Errampalli D,Saunders J,Cullen D

更新日期：2001-02-01 00:00:00

abstract：:Surface interfacial physiology is particularly important to unicellular organisms with regard to maintenance of optimal cell function. Bacterial cell surfaces possess net negative electrostatic charge by virtue of ionized phosphoryl and carboxylate substituents on outer cell envelope macromolecules which are exposed t...

journal_title：Journal of microbiological methods

authors： Wilson WW,Wade MM,Holman SC,Champlin FR

更新日期：2001-01-01 00:00:00

abstract：:New enzyme immunoassays (EIAs) for determination of specific IgG, IgA, and IgM antibody titers to Chlamydia pneumoniae were evaluated independently in three research laboratories. Specificity of the EIAs was enhanced by removing LPS from the chlamydial antigen. The performance of these EIAs was evaluated in comparison...

journal_title：Journal of microbiological methods

authors： Ossewaarde JM,Tuuminen T,Boersma WG,Sandström M,Palomäki P,Boman J

更新日期：2000-12-15 00:00:00

abstract：:Serology is commonly used for the diagnosis of acute Chlamydia pneumoniae infections and also for the diagnosis of complicated Chlamydia trachomatis infections. Furthermore, recent sero-epidemiological studies have linked C. pneumoniae infection with several diseases traditionally considered non-infectious. The object...

journal_title：Journal of microbiological methods

authors： Tuuminen T,Palomäki P,Paavonen J

更新日期：2000-11-01 00:00:00

abstract：:This study investigated the various commercially available kits and 'in-house' methods to extract DNA from Gram-negative and Gram-positive bacteria, yeast and fungal agents in commonly employed blood culture material. The main methods investigated were as follows; Qiagen QIAmp Blood kit, Roche high PCR template prepar...

journal_title：Journal of microbiological methods

authors： Millar BC,Jiru X,Moore JE,Earle JA

更新日期：2000-10-01 00:00:00

abstract：:We have developed a novel flow cytometric procedure that allows determinations of properties of protein excretion in the growth medium on a cell-by-cell basis in Saccharomyces cerevisiae. The procedure is based on labelling of a periplasmically secreted protein with antibodies conjugated to a fluorescent marker such a...

journal_title：Journal of microbiological methods

authors： Porro D,Venturini M,Brambilla L,Alberghina L,Vanoni M

更新日期：2000-09-01 00:00:00

abstract：:The efficiency of different treatments was tested to extract bacterial cells from freshwater sediment samples. The influence of sonication, density gradient centrifugation, fixation by formalin and centrifugation speed on bacterial recovery was investigated. The method developed by Smith and Azam [Mar. Microb. Food We...

journal_title：Journal of microbiological methods

authors： dos Santos Furtado AL,Casper P

更新日期：2000-08-01 00:00:00

abstract：:Host defense responses of animals and plants to pathogenic microbes are mediated in part by the release of antimicrobial substances into tissue fluids. Exploration of the antimicrobial properties of tissue fluids is often limited by their small quantity. We have developed assays of antimicrobial activity that require ...

journal_title：Journal of microbiological methods

authors： Cole AM,Wu M,Kim YH,Ganz T

更新日期：2000-07-01 00:00:00

abstract：:A rapid immunological method for trapping and selection of functionally regulated prokaryotic promoters is described. The method is based on application of a novel mini-Tn5 derived promoter probe (pUTTKZY-promoterless lacZY as a reporter and kanamycin resistance) to mutagenise a plant growth promoting fluorescent pseu...

journal_title：Journal of microbiological methods

authors： Timms-Wilson TM,Ellis RJ,Bailey MJ

更新日期：2000-06-01 00:00:00

abstract：:We have developed an anaerobic biofilm culture system. The system is inexpensive, simple to use and, unlike an anaerobic glovebox, requires no dedicated space. As a test of the system, Porphyromonas gingivalis was cultured under low oxygen (1-2 ppm) and under anaerobic conditions (

journal_title：Journal of microbiological methods

authors： Hansen MC,Palmer RJ,White DC

更新日期：2000-05-01 00:00:00

abstract：:This study was conducted to evaluate the efficacy of the mini-VIDAS Listeria monocytogenes (LMO) system (BioMérieux Vitek, Inc., Missouri, USA) for detection of L. monocytogenes in environmental and fish samples from three Portuguese cold-smoking plants and from their fresh fish suppliers. Mini-VIDAS-LMO is a fully au...

journal_title：Journal of microbiological methods

authors： Vaz-Velho M,Duarte G,Gibbs P

更新日期：2000-04-01 00:00:00

abstract：:Salmonella enterica serotype gallinarum biotype gallinarum and biotype pullorum are non-motile and pathogenic avian strains. Biotype gallinarum causes fowl typhoid and biotype pullorum is the cause of pullorum disease in chickens. The two biotypes could be differentiated based on biochemical characteristics. However, ...

journal_title：Journal of microbiological methods

authors： Kwon HJ,Park KY,Yoo HS,Park JY,Park YH,Kim SJ

更新日期：2000-03-01 00:00:00